Fig. 7: Impact of IgG FcG on the recruitment of effector functions. | Nature Communications

Fig. 7: Impact of IgG FcG on the recruitment of effector functions.

From: N-linked Fc glycosylation is not required for IgG-B-cell receptor function in a GC-derived B-cell line

Fig. 7: Impact of IgG FcG on the recruitment of effector functions.The alternative text for this image may have been generated using AI.

a Representative SPR sensorgrams of 2G9 sIgG FcG+ and FcG− binding to human FcɣRI and II/IIIa. Association and dissociation are represented as response units [RU] over time [s]. b KD values of binding of FcG+ and FcG− 2G9 sIgG to human FcɣRI and II/IIIa as determined by SPR. Bar graphs show mean, standard error, and individual data points. n = 3 biologically independent experiments. **p = 0.0036 (two-sided paired t test). c Detection of intact antigen-specific (CCP2) IgG and d antigen-specific classical complement activation of FcG+ and FcG− 2G9 sIgG determined by ELISA. Deposition of complement components C1q, C4, and C3c was detected in the presence or absence of exogenous complement (normal human serum, NHS). Bar graphs show mean, standard error, and individual data points. n = 3 technical replicates of one experiment. ns (not significant) p > 0.05, **p = 0.001 (C4), **p = 0.0009 (C1q), ****p = 0.000002 (C3c) (two-sided unpaired t tests). Source data are provided as a Source Data file.

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