Fig. 1: Characterization of hANOs during epithelial differentiation. | Nature Communications

Fig. 1: Characterization of hANOs during epithelial differentiation.

From: Human apical-out nasal organoids reveal an essential role of matrix metalloproteinases in airway epithelial differentiation

Fig. 1

A, B Representative pictures of hANOs morphology (by bright field microscopy, at 100x and 200x magnification, respectively) and candidate markers (p63, βIV-tubulin, MUC5AC, FOXJ1, and ZO-1) of hANO development (by multiphoton microscopy) at different stages of hANO differentiation (D10, D17, and D24). Time lapse microscopy videos for hANOs in different differentiation stages shown in Supplementary Movie 1 to 3; Cilia beating patterns of hANOs shown in Supplementary Movie 4. C Gel degradation and F-CHP at different stages of hANO differentiation (D10, D17, and D24). D Collagen degradation was analyzed by quantifying the ratio of F-CFP/Collagen gel signals; the Mann–Whitney test was used in comparison analysis; data present the median with interquartile range, n = 8 experiments per condition; the experiment was independently performed in three organoid lines from three different donors; ns, not significant.

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