Fig. 3: Chromatin Architecture in Primary Human Embryonic Craniofacial Tissue Suggests Interaction between HOXA Gene Cluster and Gene Desert Superenhancer on Chromosome 7. | Nature Communications

Fig. 3: Chromatin Architecture in Primary Human Embryonic Craniofacial Tissue Suggests Interaction between HOXA Gene Cluster and Gene Desert Superenhancer on Chromosome 7.

From: A distant global control region is essential for normal expression of anterior HOXA genes during mouse and human craniofacial development

Fig. 3: Chromatin Architecture in Primary Human Embryonic Craniofacial Tissue Suggests Interaction between HOXA Gene Cluster and Gene Desert Superenhancer on Chromosome 7.The alternative text for this image may have been generated using AI.

HiC of H9 human embryonic stem cells (hESCs) (a), cranial neural crest cells (CNCCs) derived from H9 hESCs (b) and CS17 primary human embryonic craniofacial tissue (c). TADs called at two different resolutions, 50 kb (light blue/light yellow) and 100 kb (blue/dark yellow). Superenhancers (for CNCCs and CS17 tissue) determined by the ROSE algorithm. CTCF ChIP-seq data from (ref. 46; GSE145327). ChromHMM chromatin states from 25-state model for H9 and H9-derived CNCCs are shown below their respective HiC interaction plots and chromatin states for CS13-CS20 and F2 human craniofacial tissue are shown below the HiC interaction plot for CS17 primary craniofacial tissue. Individual enhancer segments are yellow and orange. Inset image: 3D rendered Carnegie stage 17 human embryo demonstrate representative staging of tissue used in HiC experiments The embryo was imaged using High Resolution Episcopic Microscopy (HREM): raw data courtesy of Dr Tim Mohun (Francis Crick Institute, London, UK) and provided by the Deciphering the Mechanisms of Developmental Disorders (DMDD) program (https://dmdd.org.uk/).

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