Fig. 4: Structure variations caused by whole-genome-wide SCRaMbLE in diploid strains.
From: Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae
a SynIII rearrangements detected in diploids. Arrows indicate segments between two adjacent loxPsym sites. Arrow colors in the SCRaMbLEgram indicate the segment number in the parental chromosome, and arrow directions indicate orientation. Arrows with red borders denote segments containing centromeres. Strains with an asterisk (*) have a circular synIII as a result of SCRaMbLE. b Schematic of Nanopore sequencing analysis of chromosome changes. A deletion between II-6 and II-7 was found in JDY550. A translocation between chrIV and chrX mediated by IV-9 and X-5 was found in JDY549 and JDY598. A translocation between chrIV and chrXIII mediated by IV-1 and XIII-7 was found in JDY551, JDY597, and JDY599. A translocation between chrIV and chrVI mediated by IV-1 and VI-2 was found in JDY552. The upstream region of a loxPsym site is indicated by the color red, while the downstream region is indicated by the color blue. c A complex rearrangement event among chromosomes III, IV, and synIII in JDY550. The translocation occurred at the IV-6 site and involved synIII and chrIII. This rearrangement was confirmed by both Nanopore and Sanger sequencing. Green bars show the sequence of one typical Nanopore read. LPACS, loxPsym, and adjacent co-transformed sequence.
