Fig. 1: Improvement in resolution for AAV2 at different tilt angles.
From: Overcoming resolution attenuation during tilted cryo-EM data collection
AAV2 cryo-EM data was collected at increasing stage tilt angles (0° black bars, 10° light orange bars, 20° light blue bars, 30° green bars, 40° yellow bars, 50° dark blue bars, 60° dark orange bars) and equalized for defocus, with 7000 particles used per tilt angle. The particles were processed using an identical workflow with global resolution at the end of each processing step plotted on the y-axis in Ångstroms (Å). a Baseline processing strategy presented in ref. 15. b–h Individual steps were added to the workflow, and improvements in resolution are plotted as lines. These include (b) patch-based CTF estimation in cryoSPARC, (c) CTF refinement in RELION, (d) Bayesian polishing in RELION, (e) a second round of CTF refinement and (f) Bayesian polishing, (g) a third round of CTF refinement, and (h) Ewald curvature correction.
