Fig. 3: Improvement in resolution for apoferritin at different tilt angles.
From: Overcoming resolution attenuation during tilted cryo-EM data collection
Apoferritin cryo-EM data was collected at increasing stage tilt angles (0° black bars, 10° light orange bars, 20° light blue bars, 30° green bars, 40° yellow bars, 50° dark blue bars, 60° dark orange bars) and equalized for defocus, with 17,000 particles used per tilt angle. This figure follows the format of Fig. 1. The particles were processed using an identical workflow with global resolution at the end of each processing step plotted on the y-axis in Ångstroms (Å). a Baseline processing strategy used in ref. 15. b–h Individual steps were added to the workflow and improvements in resolution are plotted as lines.
