Fig. 4: Evaluation metrics for apoferritin at representative tilt angles. | Nature Communications

Fig. 4: Evaluation metrics for apoferritin at representative tilt angles.

From: Overcoming resolution attenuation during tilted cryo-EM data collection

Fig. 4

Evaluation metrics of the cryo-EM data and reconstructed maps for the processing strategy outlined in Fig. 3. Results for untilted (left column), 30°-tilt (middle column) and 60°-tilt (right column) are compared. a Micrographs at 1.3 µm defocus, with corresponding 2D class averages at right. b Final Euler angle distribution. c Surface sampling plot with the sampling compensation factor (SCF) value below (left) and central slice through 3DFSC, colored by resolution (right). d Half-map and map-to-model global FSC curves. e Map density with docked atomic model.

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