Fig. 7: Tomographic reconstruction of a tilt-series showing RNAP particles at the air-water interface.
From: Overcoming resolution attenuation during tilted cryo-EM data collection
a Representative slice through a tomogram showing the hole used for imaging (left) and rendering of particles within the same reconstructed tomogram viewed down the XY plane (right). b Cross-sectional view of the tomogram along entirety of the YZ plane. c Thin slices of cross-sectional views at 3 distinct locations along the YZ-plane, with the top, middle and bottom slices representing three distinct locations of the hole. Dashed boxes in (a) display the locations of each slice in (c). Particles are colored according to their positions along the Z-axis, as extracted from the metadata file. Color key is described by the scale at the bottom-right panel.
