Fig. 4: Evolution of chemosensory gene expression.
From: Evolution of chemosensory tissues and cells across ecologically diverse Drosophilids
A Expression changes mapped onto the species tree for genes belonging to the main chemosensory gene families (Grs gustatory receptors, Irs ionotropic receptors, Ors odorant receptors, CSPs chemosensory proteins including CheA and CheB family members, Obps odorant binding proteins, ppk pickpocket ion channels, Trps transient receptor potential channels). The number above each branch is the total number of expression changes (up and down) across tissue samples, with the thickness of the branch proportional to that count. The number under the gene family name corresponds to the number of 1:1 orthologs used for the analysis. B–F in situ HCR results for chemosensory genes with a species-specific gain of expression. On the left is the species tree (not to scale) with the mean normalized read counts obtained for each sample. The mean and standard error are represented by the colored dots and the vertical line, respectively, with the individual data points in gray. AN antenna, LA larva head, LE forelegs, OV ovipositor, PR proboscis. RNA in situ hybridizations on the right with the targeted tissues(s) above each column. White arrows indicate cells with species-specific receptor expression. Scale bars: 30 µm. See also Supplementary Figs. 17–18. In C and E, autofluorescence highlights the sacculus in the three species. Location of source data for this figure can be found in “Source_data.xlsx”.
