Fig. 2: 19F solution NMR results of 5FW labeled WT and YnF mutants of AppA, OaPAC, and SyPixD BLUF domains and 5FW alone in 10% D2O at 298 K.
From: Origin of the multi-phasic quenching dynamics in the BLUF domains across the species

The 19F solution NMR peaks of AppA, OaPAC, and SyPixD BLUF domains are colored red, blue and green, respectively. Their resolved peaks are marked with numbers (1, 2/2′, 3/3′), denoting three classified configurations. Peak 1 has been assigned to the WinNHin configuration, Peak 2 and 2′ have been assigned to subpopulations of the WinNHout configuration, and Peak 3 and 3′ have been assigned to subpopulations of the Wout configuration. The chemical structure of 5FW with atomic numbering is depicted in the lower panel.