Fig. 4: Target analysis results of YnF mutants in H2O buffer.
From: Origin of the multi-phasic quenching dynamics in the BLUF domains across the species
a–c columns show the target analysis results of AppA Y21F (a), SyPixD Y8F (b), and OaPAC Y6F (c) in H2O condition, respectively. The upper panels illustrate the proposed kinetic models, single rocking (a) and double rocking models (b, c), for the photoreaction cycle. The arrows depict pathways with fitted averaged lifetimes, while the symbol “×” in (a) indicates that PT2 is inaccessible within our time window for the AppA Y21F mutant. In the middle and lower panels, the species-associated differential spectra (SADS) and corresponding kinetic traces are presented; FMN* is depicted in red, FMN˙−/WH˙+ in purple, FMN˙−/W˙ in green, and FMNH˙/W˙ in blue. The inserts are the initial dynamics up to 50 ps.
