Fig. 5: 19F solution NMR results of 7FW labeled WT samples of AppA, OaPAC, and SyPixD BLUF domains and 7FW alone in 10% D2O at 298 K.
From: Origin of the multi-phasic quenching dynamics in the BLUF domains across the species
The 19F NMR spectra of AppA, OaPAC, and SyPixD BLUF domains are shown in red, blue, and green, respectively. Their resolved peaks are marked with numbers (1/1′, 2/2′, 3), denoting three classified configurations. Peaks 1 and 1′ correspond to subpopulations of the WinNHin configuration, Peaks 2 and 2′ relate to subpopulations of the WinNHout configuration, while Peak 3 represents the Wout configuration. The chemical structure of 7FW, along with atomic numbering, is illustrated in the lower panel.
