Fig. 5: CF synapse translocation is perturbed in conditional KO mice lacking Dscam in Purkinje cells.

a Table showing the depletion of Dscam on Purkinje cells, granule cells, and ION neurons in each cKO mouse. ION: inferior olive nucleus. CF: climbing fibre. cKO: conditional knockout. b Immunohistochemistry of Calbindin and vGluT2 in P30 Control and conditional KO mouse cerebella. Dotted lines and asterisks represent the pial surface and Purkinje cell soma, respectively. The molecular layer located at the upper area of the Purkinje cell layer (PCL) was divided into three areas in e; Zone I was up to 40 µm in height from the PCL; Zone II was from 40 µm to 80 µm in height; Zone III was above 80 µm in height. Scale bar, 50 µm. Quantification of the total vGluT2 puncta number (c) and the ratio of vGluT2 height to molecular layer height (d) in the molecular layer of each cKO cerebella. The numbers in parentheses are mice count. Data represent mean ± SEM; Two-way ANOVA with multiple comparisons. e Regional changes in the total number of vGluT2 puncta per 6\(\times\)10³ µm². The zone was represented in a. The numbers in parentheses are mice count. Data represent mean ± SEM; Two-way ANOVA with multiple comparisons.