Fig. 2: RNA sequencing of purified β-cells following 2-day HFD.
From: A role and mechanism for redox sensing by SENP1 in β-cell responses to high fat feeding
A β-cells from CD and 2-day HFD were isolated through fluorescence activated cell sorting (FACS) for RNA sequencing. 213 genes were identified differentially expressed (DE) genes after 2-day HFD (213 genes, n = 4 and 3 mice). No adjustments were made for multiple comparisons. B All DE genes were submitted to Metascape for functional enrichment analysis using the standard accumulative hypergeometric statistical test. Expression of DE genes on CD and HFD were normalized to z-scores by gene and colorized in circos heat map. The six most enriched pathways (PW) are shown. All DE genes were submitted to STRING database for protein-protein interaction. The related protein interactions for DE genes enriched in the six PW were highlighted as links in the circos plot. C Gene set enrichment analysis (GSEA) showed significant up- and down-regulated pathways after HFD with false discovery rate (FDR) less than 0.05. All nominal p-values were less than 0.01. Normalized enrichment score (NES) reflects the degree to which a gene set is overrepresented in a ranked list of genes. D Illustration of transcriptomic changes related to metabolism after 2-day HFD. Raw sequencing data available in the GEO repository (GSE249790).
