Fig. 4: Using TEMPy-ReFF for map composition.
From: Cryo-EM structure and B-factor refinement with ensemble representation
a Composition of 5 component maps (EMD-34227, 34229, 34230, 34235, 34236) shown in their overlapping position on the left, combined to create the composite map shown on the right. b Composite map of the Singapore grouper iridovirus capsid (EMD-34815), shown as a blue surface rendering. In order to simplify visual comparison, we masked the original map such that only density around the fitted model (PDB ID: 8HIF) is shown. The deposited composite map retains some artefacts at the borders between the, approximately circular, component maps, where the map density is less intense. This is highlighted in the insets, which also show the fitted model, coloured green. c Composite map, shown as an orange surface rendering, produced using the responsibilities computed by TEMPy-ReFF as weights for each component map. The insets show the map density with the model, again shown in green, at the same location as shown per b. Clearly, the artefacts are no longer present.
