Fig. 5: ICMTs, rhoptries and MVs are affected by ICMAP2 depletion.

a ICMTs signal is partially lost upon ICMAP2 depletion in extracellular parasites when assessed by U-ExM. On the left are representative pictures, with a white arrow pointing at the ICMTs staining. Tub tubulin. Mean ± SD and individual replicates are presented (n = 20 for each replicate). Unpaired two-tailed Student’s t-tests were performed and p value is indicated on the graph. b ICMTs seem lost upon icmap2 knock-out when assessed by EM of extracellular parasites. On the left are representative pictures, with a white arrow pointing at the ICMTs. Mean ± SD and individual replicates are presented (n = 59 for each replicate). Unpaired two-tailed Student’s t-tests were performed and the p value is indicated on the graph. c Cryo-ET reveals that ICMAP2-depleted parasites show abnormal positioning of MVs, rhoptries and ICMTs. Two typical tomograms are shown. Tomograms at two relevant cross-sections are shown on the left and the associated 3D segmentation is shown on the right. d Depletion of ICMAP2 does not affect the presence of the AV. e Depletion of ICMAP2 affects the number of rhoptries docked to the AV. f Depletion of ICMAP2 affects the number of ICMT observed inside the conoid. g Significantly shorter ICMTs are observed in ICMAP2-depleted parasites. For ICMAP2, n = 13 for each ICMT. Unpaired two-tailed Student’s t-tests were performed where ns if P > 5 × 10⁻². For panels (d)–(g), 20 independent tomograms per condition were quantified. For panels (d), (e) and (g), the ΔKu80 control data are the same as the ones presented in Fig. 3. h Cryo-ET reveals, in one tomogram of ICMAP2-depleted parasite, the presence of two AVs docked at the plasma membrane. Tomograms at four relevant cross-sections are shown on the left and the associated 3D segmentation is shown next to it. A top view is shown on the right panel, highlighting the presence of two 8-fold rosettes.