Fig. 4: Longer transcripts are enriched better in the transcriptome. | Nature Communications

Fig. 4: Longer transcripts are enriched better in the transcriptome.

From: Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

Fig. 4

a Scatterplots showing the percentage of reads belonging to the unenriched 4997 transcripts of different lengths (200–400 bp, 400–600 bp, 600–1000 bp, >1000 bp) in bulk sequencing (X-axis) and in adaptive sampling (Y-axis), after enrichment for 319 genes. b Scatterplots showing the percentage of reads belonging to the rest of the transcriptome with different lengths (200–400 bp, 400–600 bp, 600–1000 bp, >1000 bp) in bulk sequencing (X-axis) and in adaptive sampling (Y-axis), after depleting the top 150 genes. c, d IGV plots showing the location and read count of newly identified transcripts through adaptive sampling by depleting all known transcripts. The Waston strands are labeled blue, and Crick strands are labeled brown.

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