Fig. 4: In vivo promoted ferroptosis of Gi-F-CAA by AEB effect.
From: In vivo assembly enhanced binding effect augments tumor specific ferroptosis therapy
a Viability of EJ cells after treated with Gi-F (pH = 7.4), Gi-F-SA (pH = 7.4), NGi-F-CAA (pH = 7.4), NGi-F-CAA (pH = 6.5), Gi-F-CAA (pH = 7.4) and Gi-F-CAA (pH = 6.5) at different concentrations for 48 h (n = 3 experimental repeats). Experiment was independently repeated three times with similar results. b Confocal laser scanning microscopy (CLSM) images of EJ cells after treated with Gi-F-CAA (pH = 7.4) and Gi-F-CAA (pH = 6.5, 40 μM). Scale bar: 10 μm. c Fluorescence binding distribution images of NBD labelled Gi-F-CAA (pH = 6.5, 40 μM) and GPX4 in EJ cells. Anti-GPX4 antibody was used to label GPX4. Scale bar: 10 μm. d Intracellular glutathione peroxidase 4 (GPX4) activities of EJ cells after treated with PBS (pH = 7.4), NGi-F-CAA (pH = 7.4), Gi-F-SA (pH = 7.4), Gi-F-CAA (pH = 7.4) and Gi-F-CAA (pH = 6.5, 40 μM). The GPX4 activities of PBS group was normalized as 1 (n = 3 experimental repeats). Experiment was independently repeated three times with similar results. e Intracellular iron levels of EJ cells after different treatment by FerroOrange fluorescence staining. Scale bar: 10 μm. f Intracellular ROS levels of EJ cells after different treatment by DCFH-DA fluorescence staining. Scale bar: 10 μm. g Intracellular lipid hydroperoxide (LPO) levels of EJ cells after different treatments by C11-BODIPY staining. Scale bar: 10 μm. h Bio-TEM images of EJ cells after treated with PBS (pH = 7.4) and Gi-F-CAA (pH = 6.5, 40 μM). The red box dashed region indicates the mitochondria. Scale bar: 10 μm. Data were presented as mean ± SD. P-values were performed with one-way ANOVA followed by post hoc Tukey’s test. Data were expressed as mean ± SD. Source data are provided as a Source Data file.
