Table 3 CSNK1A1sgRNA design

From: Selective CK1α degraders exert antiproliferative activity against a broad range of human cancer cell lines

Name

Sequence (5’–3’)

SM148.CRBN.g3 spacer

UGUAUGUGAUGUCGGCAGAC

CAGE1215.CSNK1A1.g1 spacer

UUCUAGUCGCCGAGAUGACA

CAGE1215.CSNK1A1.g7 spacer

UUUACCUUUAGCCCUUGCCA

Forward indexing primer (5’–3’)

AATGATACGGCGACCACCGAGATCTACAC(6 bp index)ACACTCTTTCCCTACACGACGCTCTTC

Reverse indexing primer (5’–3’)

CAAGCAGAAGACGGCATACGAGAT(10 bp index)GTGACTGGAGTTCAGACGTGTGCTC

SM148.hCRBN.DS.F2

ctacacgacgctcttccgatctGCAGAGAGTGAGGAAGAAGATGA

SM148.hCRBN.DS.R2

cagacgtgtgctcttccgatctGCCCATGTCCTCATCCACAA

CAGE1215.CSNK1A1.F

ctacacgacgctcttccgatctCACCAAATAGTGTTCCCTCCTCA

CAGE1215.CSNK1A1.R

cagacgtgtgctcttccgatctTGGGTACCAGCTTACTGTCTCT

Back to article page