Fig. 5: PixON and ON alpha RGCs receive excitatory input from the same BCs.
a En-face view of filled PixON and ON alpha RGCs (green) imaged with 2-photon microscopy in the CCK-ires-Cre/Ai14 mouse line, which labels T6 BCs (red). Inset shows laser burn marks used as fiducial markers during SBFSEM alignment (see “Methods”). b PixON and ON alpha SBFSEM reconstructions of the tissue volume indicated by the white rectangle in (a). c Example reconstruction showing a T6 BC (semi-transparent gray mesh) forming ribbon synapses (red) onto a PixON dendrite (purple) and an ON alpha dendrite (brown). Reconstruction is taken from the approximate location indicated by the gray rectangle in (b) and rotated for better visibility of synapses. d Reconstruction of a T6 BC ribbon synapse onto a PixON dendrite (synapse #1 from (c)). e SBFSEM slices used to identify the ribbon synapse from (d) (red arrow). Top and bottom slices are situated on the same XY location, but the bottom slice is 50 nm deeper in Z. f, g, same as (d, e) but showing a T6 BC ribbon synapse onto an ON alpha dendrite (synapse #2 from (c)). h En-face (top) and orthogonal view (bottom) of BC types (T6-T9) presynaptic to the PixON RGC. i Same as (h) but for BCs presynaptic to the ON alpha RGC. j The proportion of synapses formed by each BC type onto the PixON (n = 86 synapses) and the ON alpha (n = 50 synapses) RGCs. Differences in the proportion of BC type between PixON and ON alpha were not significant. p > 0.05, two-sided two-proportions z-test with Holm-Bonferroni correction for multiple comparisons. Source data are provided as a Source Data file. Data come from one reconstruction.
