Fig. 3: Mapping of DREB2A binding sites in Med25-ACID using NMR. | Nature Communications

Fig. 3: Mapping of DREB2A binding sites in Med25-ACID using NMR.

From: Molecular switching in transcription through splicing and proline-isomerization regulates stress responses in plants

Fig. 3

A 15N-HSQC spectra of 15N labeled Med25-ACID in complex with ABS (red), RIM (blue). and ABS-RIM (yellow). Zoom: residues affected differently depending on the DREB2A fragment. B 15N,HN CSPs of Med25-ACID induced by ABS (top), RIM (middle), and ABS-RIM (bottom). Red and blue background: regions forming the ABS and the RIM binding sites, respectively. Bars are colored according to their residue color in panels D and E. *Residues highlighted in panel A. C Same as B, but tracing HSQC relative peak intensities as a function of DREB2A fragment concentration (dark to light). Molar ratios for the final intensity level (light color) are provided in the top right corner. D Mapping of ABS induced Med25-ACID CSPs on the AF2 structure. E Mapping of Med25-ACID CSPs induced by the bivalent ABS-RIM fragment. Blue spheres: RIM induced additional CSPs. D and E are colored according to significance levels of 0.1 (light) and 0.2 ppm (dark). Source data are provided as a Source Data file.

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