Table 1 Thermodynamic parameters of DREB2A interaction with Med25-ACID at 25 °C

From: Molecular switching in transcription through splicing and proline-isomerization regulates stress responses in plants

DREB2A

N-value

Kd (nM)

∆H (kJ/mol)

−T∆S (kJ/mol)

∆G (kJ/mol)

 151–335

1.10 ± 0.06

540 ± 40

−52.3 ± 0.8

16.5 ± 1.0

−35.8 ± 0.2

 195–276

1.09 ± 0.02

450 ± 30

−51.2 ± 0.6

15.0 ± 0.7

−36.2 ± 0.2

 234–335

1.0 ± 0.2

440 ± 60

−58 ± 1

22 ± 2

−36.3 ± 0.3

 234–276 (ABS-RIM)

0.98 ± 0.08

510 ± 20

−50.7 ± 0.3

14.8 ± 0.4

−35.9 ± 0.1

 234–272

0.97 ± 0.05

1800 ± 400

−43 ± 3

10 ± 4

−32.8 ± 0.5

 234–256 (ABS)

0.86 ± 0.03

6000 ± 200

−54.0 ± 0.5

24.2 ± 0.6

−29.8 ± 0.1

 255–276 (RIM)*

–

5,000,000

–

–

–

 195–335 ∆244–276

1.02 ± 0.09

5400 ± 600

−59 ± 2

28 ± 2

−30.1 ± 0.3

DREB2A 195–276

     

 L237A

1.03 ± 0.06

2400 ± 300

−22.9 ± 0.7

−9.2 ± 0.9

−32.1 ± 0.3

 V239A

0.95 ± 0.01

2000 ± 200

−42 ± 1

10 ± 1

−32.5 ± 0.3

 D241A

1.07 ± 0.06

2000 ± 700

−37 ± 7

5 ± 7

−32.5 ± 0.9

 Y242A

−

NB

−

–

−

 W244A

1.0 ± 0.1

1900 ± 200

−47 ± 1

14 ± 2

−32.7 ± 0.3

 P245A**

1.26 ± 0.01

780 ± 60

−43.4 ± 0.8

8.8 ± 0.8

−34.9 ± 0.2

 F259A

1.2 ± 0.1

550 ± 50

−58.8 ± 0.9

23 ± 1

−35.8 ± 0.2

 E263P

1.00 ± 0.06

580 ± 40

−61.2 ± 0.8

25.5 ± 1.0

−35.6 ± 0.2

 F274A

0.86 ± 0.02

660 ± 90

−62 ± 2

27 ± 2

−35.3 ± 0.3

Med25-ACID

     

 R568A

0.90 ± 0.01

3,100 ± 700

−46 ± 4

14 ± 5

−31.5 ± 0.5

  1. All DREB2A WT ITC data represent the results of two or more replicates. Shown values represent mean and standard deviations from replicates. *Affinity determined using NMR, **P245A variant was made in the DREB2A234-276 fragment, NB: no detectable binding. The table is provided as a Source Data file.
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