Fig. 2: MAP2 interactions at the ribosomal tunnel exit. | Nature Communications

Fig. 2: MAP2 interactions at the ribosomal tunnel exit.

From: Methionine aminopeptidase 2 and its autoproteolysis product have different binding sites on the ribosome

Fig. 2

The central panels show an overview of HsMAP2 and CtMAP2 binding to the PTE. MAP2 is shown in red and the insert domain is indicated by the dashed box. a The core domain of HsMAP2 electrostatically interacts with the 28S and 5.8S rRNA through several charged residues. b The insert domain of HsMAP2 is fixed on 28S rRNA H53 (R395 stacks onto C2526) and binds specifically to H59 at U2708. Unlike CtMAP2, HsMAP2 tightly interacts with H59, which undergoes large conformational changes upon MAP2 binding. c Main contact points between CtMAP2 and the ribosome include interactions of R361 with the 28S rRNA. d Hydrophobic interactions involving leucine residues between tunnel exit protein uL23 and CtMAP2.

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