Fig. 6: AtIMA3 is required for nitrogen homeostasis depending on nitrogen–Fe balance. | Nature Communications

Fig. 6: AtIMA3 is required for nitrogen homeostasis depending on nitrogen–Fe balance.

From: IMA peptides regulate root nodulation and nitrogen homeostasis by providing iron according to internal nitrogen status

Fig. 6

a, b Shoot FW (n = 17–40 plants) and chlorophyll contents (n = 6 independent pools of shoot derived from three plants) of WT, Atima3, and Atima1/2/3 plants. Plants were grown on agar plates with 1/2 MS medium containing different concentrations of nitrate for 14 d. c–e Effects of different nitrogen–Fe ratios on plant growth, nitrate contents (n = 6 independent pools of shoot derived from three plants), and chlorophyll contents (n = 6 independent pools of shoot derived from three plants). Plants were grown on agar plates with 1/2 MS medium containing different concentrations of nitrate and Fe for 14 d. Scale bar, 1 cm. Centerlines in the boxplots show the medians, and box limits indicate the 25th and 75th percentile. The whiskers go down to the smallest value and up to the largest. Scatterplots show individual biological replicates as dots. In a, b, d, e, different letters indicate statistically significant differences (P < 0.05, two-way ANOVA followed by multiple comparisons).

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