Fig. 3: RvT4 increases phagocytosis and chemotaxis.
From: Resolvin T4 enhances macrophage cholesterol efflux to reduce vascular disease
a oxLDL-loaded monocyte-derived macrophages were incubated for 30 min with vehicle or 1 nM RvT4. Apoptotic HL-60 cells stained with pHrodo SE were then added, and phagocytosis measured. n = 5 per group; Statistical differences were evaluated using repeated measures 2-way ANOVA. b Area under curve (left) and rate of apoptotic cell uptake (right) were calculated using GraphPad Prism software. Mouse bone marrow-derived oxLDL-loaded macrophages were incubated with vehicle or RvT4 (1 or 10 nM). A.U. = arbitrary units. Chemotaxis was assessed using c vehicle, d MCP-1 or e ATP as chemoattractants, and migrated cells enumerated. n = 3–4 per group; Statistical differences were evaluated using 1-way ANOVA. Aortas of ApoE-/- mice fed a Western-style diet for 8 weeks were isolated and incubated with vehicle of RvT4. f At the end of the incubation, cells that had migrated out of the tissue onto the well were collected and enumerated. g Aortas were collected, cells were liberated and the percentage CD64 + F4/80+ cells in total cell count were determined. n = 4–5 per group; Statistical differences were evaluated using unpaired t-test. Unless specified, error bars, mean ± s.e.m.; all ANOVA results were corrected for multiple comparisons by controlling the False Discovery Rate using the two-stage step-up method of Benjamini, Krieger and Yekutieli. Results are from two separate experiments.
