Fig. 2: Cryo- EM structure of Mycobacterium smegmatis 70S ribosome RafH complex. | Nature Communications

Fig. 2: Cryo- EM structure of Mycobacterium smegmatis 70S ribosome RafH complex.

From: Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH

Fig. 2: Cryo- EM structure of Mycobacterium smegmatis 70S ribosome RafH complex.

The overall architecture of the 70S ribosome RafH complex is shown in the mRNA entry site (a) and mRNA exit site (b) by a rotation through a diagonal axis. The SSU 16S rRNA (khaki), SSU r-proteins (dark golden), RafH (maroon), tRNA (pink), the LSU 23S rRNA and 5S rRNA (cornflower blue), LSU r- proteins (royal blue), bS1 (dark salmon) and uS2 (orange) are labeled. The single particle reconstruction data processing summary is shown in Supplementary Fig. 3, gold standard FSC and local resolution of final maps are shown in Supplementary Fig. 4, The cryo- EM maps for individual r-proteins, bS1 and uS2 and E-site tRNA and their model is shown in Supplementary Fig. 5, a full RafH model is shown in Supplementary Fig. 7. c The RafH NTD cryo- EM density (left panel) and model in ribbon (right panel), the top panel is rotated by 180° along X-axis, and shown in the bottom panel, the secondary structures are labeled. d The cryo- EM density in mesh and model in stick style corresponds to RafH linker region residues, 111–124 (maroon), and a-SD (anti-Shine Dalgarno sequence) region of 16S rRNA nucleotides, 1518–1522 (khaki), are shown. For more clarity, an animation is provided in Supplementary Movie 1.

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