Fig. 4: Detection of agenT-797 at multiple timepoints during patient hospitalization. | Nature Communications

Fig. 4: Detection of agenT-797 at multiple timepoints during patient hospitalization.

From: A phase 1/2 clinical trial of invariant natural killer T cell therapy in moderate-severe acute respiratory distress syndrome

Fig. 4: Detection of agenT-797 at multiple timepoints during patient hospitalization.The alternative text for this image may have been generated using AI.

Cellular composition of nucleated material in blood and BAL. Whole blood frozen at −80 °C and sent to MiNK for evaluation. BAL samples collected at bedside and immediately separated into two aliquots, then one aliquot was immediately frozen at −80 °C and sent to MiNK for evaluation. The remainder of the sample was sent to the clinical lab for processing. Cell counts and differentials were determined and closely correlated. A Persistence of agenT-797 in blood. Cohort-level peripheral persistence of agenT-797 Quantification of agenT-797 in patient PBMC by digital PCR based on genetic markers unique to donor material. Each line represents data from one patient. The red line indicates patients treated with agenT-797 and on  VV-ECMO. B EUA patient. Standard cytology cell counts in BAL and differentials. C EUA patient. Detection of agenT-797 in peripheral blood (black circles) and BAL (red circles) pre-infusion and at various timepoints post infusion with agenT-797. Data for four separate agenT-797-specific markers measured at each timepoint in blood and BAL samples shown as mean with standard deviation (SD). Detection and quantification of agenT-797 was by detection of agenT-797-specific single nucleotide polymorphisms (SNPs) using a digital droplet (dd) PCR-based assay to detect genetic chimerism (Neogenomics, CA). Pre-infusion timepoint for BAL: ID -4, and for blood: ID 0.

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