Fig. 3: NG2⁺Runx1⁺ AGM cells display a sclerotome-derived vSMC transcriptomic profile.

a t-SNE plots showing the distribution of Runx1 and Acta2 expression in NG2⁺Runx1⁺ cells in the WT E11 AGM after excluding all other (grey) cells found in the Fig. 2a. b Zoom into NG2⁺Runx1⁺ cluster (black rectangle) shows the presence or the absence of Acta2. c Heatmap showing the expression of Cspg4 and Runx1 and 15 selected genes out of 25 top significantly upregulated genes in WT NG2⁺Runx1⁺Acta2⁺ cells (upper half) and NG2⁺Runx1⁺Acta2^- cells (bottom half) at single cell level; *Runx1 potential target genes. Pdgfra and Ptn genes were next added to inform their expression in both populations. Barplot of fold enrichment for selected GO biological processes significantly overrepresented in genes significantly upregulated in both d WT NG2⁺Runx1⁺Acta2⁺ and e NG2⁺Runx1⁺Acta2^- cells. f t-SNE of WT E11 AGM cells, overlaid with principal pseudotime curve inferred by Slingshot, predicting a lineage from NG2⁺Runx1⁺Acta2^- cells to NG2⁺Runx1⁺Acta2⁺ cells. g WT NG2⁺Runx1⁺cells arranged in pseudotime (x-axis) based on the inferred curve. Y-axis represents log normalised gene expression.