Fig. 1: HCV and VSV infection upregulates cellular FUT8 mRNA and protein expression.
a Analysis of FUT family mRNA expression in Huh7 cells infected with HCV (MOI = 0.1) for the indicated time using RT-qPCR (n = 3 per group per study). b Lectins (AAL and AOL) and immunoblot analysis of core fucosylation and FUT8 in lysates of Huh7 cells infected with HCV (MOI = 0.1) for the indicated time. c RT-qPCR analysis of Fut family mRNA expression in hepatocytes of ICR4R+ mice infected with HCV (1 ×106 copies per mouse) in vivo for 40 d (n = 3 mice per group per study). The figure is created with Biorender.com. d Immunoblot analysis of FUT8 in lysates of Huh7 cells transiently transfected with eukaryotic expression plasmids encoding each of the ten HCV proteins. e FUT8 promoter activity in Huh7 cells transiently co-transfected with pGL3 luciferase reporter system and eukaryotic expression plasmids of individual HCV genes. Luciferase activities were analyzed as fold induction (vs. pcDNA3.1 empty vector group) (n = 3 per group per study). f RT-qPCR analysis of FUT8 mRNA expression in Huh7 cells transiently transfected with His-vector or His-E2 plasmids at the indicated concentrations (n = 3 per group per study). g Immunoblot analysis of FUT8 and core fucosylation in lysates of Huh7 cells transiently transfected with indicated plasmids at the indicated concentrations. h RT-qPCR analysis of FUT8 mRNA expression in HEK293T infected with VSV (MOI = 0.1) for the indicated time (n = 3 per group per study). RT-qPCR analysis of FUT8 mRNA expression (i) and lectin and immunoblot analysis of FUT8 and core fucosylation (j) in lysates of HEK293T cells transiently transfected with the indicated plasmids for the indicated time. Data in all quantitative panels are presented as mean ± SD. Data are representative of three independent experiments. Data are normalized based on human GAPDH for a, e, f, h, i and mouse Gapdh for c. Two-tailed unpaired Student’s t test was used to assess the statistical difference in a and h (vs. 0 h.p.i.), c (vs. Mock), e and i (vs. pcDNA3.1), f (vs. 0 μg). Source data are provided as a Source Data file.
