Fig. 2: Analysis of transduction and interference induced by PICMI₁₁₅.

a To determine transduction frequencies, lysates containing viral particles with the chloramphenicol-resistant (Cm^R)-encoding PICMI₁₁₅ were used at the specified titer (PFU ml^-1) to infect both a derivative lacking the full PICMI₁₁₅ (ΔPICMI₁₁₅) and V511 hosts. Transductants (TFU ml^-1) were selected on chloramphenicol. Bar charts show the mean values ± standard deviation (SD) with n = 3 independent replicates depicted by individual dots. The average TFU/PFU ratio is indicated for each strain. b The integration of PICMI₁₁₅-Cm^R at the end of the fis gene was confirmed by PCR. Images are representative of two independent experiments. Uncropped gel is provided as a Source Data file. c To investigate interference with the reproduction of the helper phage, the wild-type V115 strain, ΔPICMI₁₁₅, and two clones (c1 and c2) of transductants carrying PICMI₁₁₅-Cm^R, were infected with Φ115pure. The bar charts depict the phage titer at 0 and 60 min post-infection, with values shown the mean ± SEM from three independent experiments (depicted by individual dots). At 60 min, the null hypothesis (H0) stating that the values are similar across categories cannot be rejected. The results of the t2 Wilcoxon test yielded a p-value of 0.9957. Additionally, ANOVA analysis with an F-value of 4.227 and a p-value of 0.7419 further supports the acceptance of H0. The Tukey-HSD test for every pair did not reveal any significant differences (p > 0.05).