Fig. 8: Transcriptomic analysis of macrophages expressing the pathogenic NLRP3 p.D303N variant.
A Gene expression profile of Nlrp3−/− immortalized macrophages (iMos) treated for 16 h with or without doxycycline (1 µg/ml) to induce the expression of the human NLRP3 p.D303N variant, in the absence or presence of MCC950 (10 µM). The gray boxes on the right highlight some of the genes or pathways identified in each trend. The data represented in the graphics are derived from four independent experiments. B Relative expression of glycolysis genes from iMos treated as described in A, but expressing either wild-type NLRP3 or the p.D303N variant. The data are represented as circles, where the size indicates the fold change of doxycycline treated cells vs untreated cells, and doxycycline with MCC950 treatment vs doxycycline, and the color represents the t-test two-sided –Log10 p-value (the dotted line in the color scale represents p = 0.05). The graphics represent data from three to four independent experiments. C Gene ontology for biological process enrichment analysis of differentially expressed genes from Nlrp3−/− iMos treated as described in A. The data are represented as circles, where the size indicates the gene count for that particular process, and the color represents the –Log10 p-value calculated with one-sided Fisher’s Exact test with Benjamini–Hochberg correction (the dotted line in the color scale represents p = 0.05). The graphics represent data from four independent experiments. Source data are provided as a Source Data file.
