Fig. 4: Enhanced prostaglandin signaling in males regulates McSC migration.
A Volcano plot of differentially upregulated genes in female (left) and male (right) mice. Genes that belong to Acute Inflammatory Response are highlighted. B Expression level of Ptgs2 in all immune cell subclusters described in Fig. 3B. C Log-fold gene expression changes in cyclooxygenases and select prostaglandin synthesis factors following UVB irradiation in males and females, from bulk mRNA sequencing data. *, **, *** indicates p.adjusts (apeglm shrinkage) are lower than 0.05, 0.01, 0.001, respectively. D Antibody staining for Ptges2, Ptges3, Ptger2, co-stained with F4/80 and DAPI on control skin collected at day 3. Co-localizations are indicated by circle. n = 3 males. E Schematic of Rosa26-CreER; Ptgs2f/f mouse model, created with BioRender.com. F Representative images of McSC migration and quantification from Cox-2 global knockout mice compared to controls. n = 10 male mice for control (Ctrl), n = 14 male for knockout (KO). Ptgs2f/+ or Ptgs2+/+ were used as controls. Statistics: significance calculated by Welch’s t test. Error bar: SEM. Scale bar: 100 µm.
