Fig. 4: The blocking potency between TTX(S) and TTX(C) on HEK-Nav1.7 cells.
a–f Representative traces (top) and quantitative analyses (bottom) of HEK-Nav1.7 currents under the conditions of No-TTX (a, b), 1 μM TTX(C) (c, d), and 1 μM TTX(S) (e, f). Inset in (c), a schematic image showing the protocol of step-wise voltage-clamp from −80 mV to +80 mV in 10 mV increments. g–i Quantitative analyses of concentration-dependent Nav1.7 currents evoked by membrane depolarization from −80 mV to 0 mV in the presence of TTX (S) (g) and TTX (C) (h). For a comparison of these two curves, see (i). Note: The IC50s of TTX (S) and TTX (C) were approximately 2.6903 nM and 3.7869 nM, respectively. The number of cells in each group is shown in brackets in (g–i). Error bars in b, g–i indicate mean ± SEM. TTX(S) indicates synthetic TTX; TTX(C) indicates commercialized TTX from Tocris Bioscience Inc.
