Table 1 Cryo-EM and refinement statistics of mono-pilus and tri-pilus
From: Two distinct archaeal type IV pili structures formed by proteins with identical sequence
Parameter | Mono-pilus | Tri-pilus |
|---|---|---|
Data collection and processing | ||
Voltage (kV) | 300 | 300 |
Electron exposure (e− Å−2) | 50 | 50 |
Pixel size (Å) | 1.08 | 1.08 |
Particle images (n) | ||
Shift (pixel) | 6 | 15 |
Helical symmetry | ||
Point group | C1 | C1 |
Helical rise (Å) | 4.95 | 15.80 |
Helical twist (°) | 104.80 | −39.65 |
Map resolution (Å) | ||
Map:map FSC (0.143) | 3.9 | 3.5 |
Model:map FSC (0.5) | 4.0 | 3.6 |
d99 | 4.1 | 3.8 |
Refinement and Model validation | ||
Ramachandran Favored (%) | 98.5 | 93.8 |
Ramachandran Outliers (%) | 0.0 | 0 |
Real Space CC | 0.86 | 0.86 |
Clashscore | 12.2 | 9.3 |
Bonds RMSD, length (Å) | 0.006 | 0.005 |
Bonds RMSD, angles (°) | 0.969 | 0.967 |
Deposition ID | ||
PDB (model) | 8TIF | 8TIB |
EMDB (map) | EMD-41286 | EMD-41283 |
