Fig. 3: GmCRY1b negatively regulates leaf senescence partially through the DELLA proteins. | Nature Communications

Fig. 3: GmCRY1b negatively regulates leaf senescence partially through the DELLA proteins.

From: The mechanism of low blue light-induced leaf senescence mediated by GmCRY1s in soybean

Fig. 3: GmCRY1b negatively regulates leaf senescence partially through the DELLA proteins.

a Leaf senescence phenotypes of wild-type TL1 cultivar, Gmrgas-dm/GmCRY1b-YFP-1 transgenic plants and the corresponding parental lines under long-day conditions for 25 days. Scale bar, 5 cm. Cotyledon senescence index (b) and unifoliate leaf senescence index (c) as in (a). The cotyledon and unifoliate leaf senescence index was calculated at the age of 15 days and 25 days, respectively (n ≥ 20 biological replicates). d Chlorophyll content in the unifoliate leaf at the indicated leaf age as in (a). Values are means ± SD (n = 5 biologically independent plants), *P < 0.05, **P < 0.01 by two-tailed t-test. Relative transcript levels of senescence marker genes GmSAG13 (e) and GmSAG113 (f) in the unifoliate leaf at the leaf age of 25 days as in (a). Values are mean ± SD (n = 3 biological replicates). The lowercase letters indicate significant differences (One-way ANOVA with two-sided Tukey test at a significance level of 0.05). Source data are provided as a Source Data file.

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