Fig. 8: Analysis of the processes and mechanisms of wound healing for various treatments.
From: Programmed microalgae-gel promotes chronic wound healing in diabetes
a HIF-1α expression, (b) ROS content, (c) EGF expression, and (d) VEGF expression on day 6 under various treatments. The scale bar is 100 μm. Each experiment was repeated independently three times with similar results. e–h Quantitative investigation of HIF-1α, ROS, EGF, and VEGF expression on day 6 in response to various treatments. The data are presented as the mean ± s.d. (n = 5 independent mice). Statistical differences were analyzed by Student’s two-sided t-test. Double immunofluorescence staining of (i) α-SMA (yellow) and CD31 (green) and (j) the fibroblast markers vimentin (green) and collagen (red) in response to various treatments on day 6. Scale bar, 100 μm. Each experiment was repeated independently three times with similar results. k–m Quantitative investigation of CD31 expression, neovascularization, and collagen I deposition on day 6 in response to various treatments. The data are presented as the mean ± s.d. (n = 5 independent mice). Statistical differences were analyzed by Student’s two-sided t-test.
