Fig. 9: Regulation of macrophage polarization and the immune microenvironment by HEA@Gel in vivo.
From: Programmed microalgae-gel promotes chronic wound healing in diabetes
a Double immunofluorescence staining of CD206 (green) and CD86 (pink) on day 6 in response to various treatments. Scale bar, 100 μm. Each experiment was repeated independently three times with similar results. b Analysis of the ratio of M2 to M1 macrophages in response to various treatments by flow cytometry on day 6. Each experiment was repeated independently three times with similar results. In vivo wound concentrations of (c) TNF-α, (d) IL-1β, (e) IL-6, (f) CXCL-1, (g) IL-4, and (h) IL-10 after 3 and 6 days of treatment. i White blood cell count, (j) lymphocyte count, and (k) neutrophil percentage (gran%) in peripheral blood. The data are presented as the mean ± s.d. (n = 5 independent mice). Statistical differences were analyzed by Student’s two-sided t-test.
