Fig. 3: Quantification of the cAMP content in the purified CNBDs of HCN1 and HCN2 subtypes.
From: A high affinity switch for cAMP in the HCN pacemaker channels
Representative absorbance profiles, measured at λ = 254 nm, of cAMP molecules released by (a) HCN1 CNBD ΔC-term and CNBD ΔDE proteins (black and red lines, respectively) and (b) HCN2 CNBD ΔC-term and CNBD ΔDE proteins (black and red lines, respectively) after boiling. Mean molar ratio [protein]:[cAMP] ± SEM of 3 independent experiments are reported in the main text. Calibration curves are shown in the inset (Arb. units: arbitrary units).
