Fig. 3: Activating adaptors NINL and BICDL2 regulate dynein-mediated BCV disintegration.

Time-lapse microscopic analyses of Shigella infection of cells subjected to RNA interference of non-targeting control (siRNA Neg) versus depletion of dynein activating adaptors were performed to examine the A time of BCV disassembly and B Shigella escape time. n > 50 infected cells in three independent replicates in each condition. The data were presented in the descending order of the average times determined in the respective assays (left to right). The bars (magenta) represent the mean and two-tailed Welch’s t-tests were performed. (The colors of the x-axis labels indicate the statistical significance - red: ****p < 0.0001; orange: ***p < 0.001; green: **p < 0.01; blue: *p < 0.05; black: non-significant). C Analysis of the fates of individual Shigella BCVs in control HeLa cells and cells subjected to depletion of the subset of dynein activating adaptors that showed the strongest effects determined by the analyses in A, B. The bars represent mean ± SEM of at least two independent replicates in each condition. D Analysis of the recruitment of LC3 to intracellular Shigella in infected cells at 45 min-post infection in cells subjected to RNA interference of non-targeting control (siRNA Neg) versus depletion of the subset of dynein activating adaptors. n > 60 infected cells in three independent replicates in each condition. The bars (magenta) represent the mean and two-tailed Welch’s t-tests were performed. (The colors of the x-axis labels indicate the statistical significance – orange: ***p < 0.001; green: **p < 0.01). Source data are provided as a Source Data file.