Fig. 5: Deep sequence assembly of filoviruses isolates used.

a Coverage of assembled EBOV isolates’ sequences: isolate colour-coding shown in (b). Vertical dashed lines show sites of single nucleotide polymorphisms (SNPs) against the Map Seq. b EBOV genome schematic (top). SNPs against EBOV_VC are depicted as asterisks; both are within protein coding regions (coloured boxes). Assembled consensus sequences (below). Passaged isolates are depicted as cartoons; either in vitro (culture plate) or in vivo passage in bats (blue bats: dam and foetus). Sequence regions with SNPs and corresponding amino acid (aa) translations (coloured boxes below nucleotides) are highlighted within boxes. The purple box shows the non-synonymous aa substitutions (dN) arisen during in vivo passaging. c Coverage of assembled MARV isolates’ sequences: isolate colour-coding shown in d. Vertical dashed lines show SNPs against MARV_VC. d Schematic of MARV genome (top) and SNPs detected against MARV_VC (as above), shown as asterisks in black for synonymous changes (dS) or in purple for dN. Dots show SNPs that are unique against MARV Map Seq. Asterisks show SNPs positions in each gene and purple boxes enclose the residue changes. e dN and dS changes of the MARV passaged isolates against MARV_VC (colour-coding shown in d). Map Seq mapping sequence, VC original human isolate passaged in Vero cell, MI VC isolate passaged twice in AFBs kidney cells (MoKi) and used as inoculum for in vivo infection, D1 re-isolated virus from either kidney (EBOV) or placenta (MARV) of infected AFBs, O1 re-isolated EBOV from liver of infected foetus, D1V1 D1 isolate passaged in Vero cells once, and D1V2 = twice, D1M D1 isolate passaged in MoKi cells. Source data are provided as a Source Data file.