Fig. 1: The IDR and HELICc domain-containing protein, HRDE-2, localizes to SIMR foci.

a Medium and high-confidence protein interactions identified by a SIMR-1 yeast two-hybrid screen. See Supplementary Data 1 for a complete list of interacting proteins. b Graph displaying disorder tendency for the HRDE-2 protein sequence. The disorder prediction was made using PONDR (http://www.pondr.com) with VSL2 and VSL3 parameters. Regions with PONDR scores of greater than 0.5 indicate disorder and regions with PONDR score less than 0.5 indicate order. The bold line from a.a. 185 to 305 indicates the longest predicted disordered region of HRDE-2. Below, a domain diagram for HRDE-2 shows that it contains an N-terminal HELICc domain and a C-terminal intrinsically disordered region. c Live imaging of SIMR-1::mCherry::2xHA (magenta); HRDE-2::2xTy1::GFP (yellow) hermaphrodites, showing that SIMR-1 and HRDE-2 colocalize. At least ten individual germlines were imaged. Scale bars, 5 μM. d Confocal imaging of a SIMR-1::mCherry::2xHA MUT-16::3xMyc; HRDE-2::2xTy1::GFP in a dissected day-one adult germline, using antibodies against GFP, HA, and Myc. Insets, at right, show visual examples of SIMR-1 proximity to HRDE-2 compared to MUT-16 proximity to HRDE-2. All images are a single focal plane. At least five individual germlines were imaged. Scale bar, 5 μM. e Box plot showing distance between the centers of fluorescence for HRDE-2, SIMR-1, and MUT-16 granules. 24 individual granules from 4 germ cells of 2 animals were used for quantification. Measurements for each of the 24 granules are shown as individual dots. Bolded midline indicates median value, box indicates the first and third quartiles, and whiskers represent the most extreme data points within 1.5 times the interquartile range, excluding outliers. Two-tailed t-tests were performed to determine statistical significance and p values were adjusted for multiple comparisons. See Methods for detailed description of quantification methods. Source data are provided as a Source Data file.