Fig. 2: The germline-expressed nuclear Argonaute HRDE-1 interacts with HRDE-2.
From: HRDE-2 drives small RNA specificity for the nuclear Argonaute protein HRDE-1
a Peptide counts for proteins identified by the IP-mass spec of wild-type, SIMR-1::mCherry::2xHA, and HRDE-2::2xTy1::GFP strains, replicate 1. Peptide counts for two additional replicates can be found in Supplementary Fig. 2c. Anti-HA conjugated beads were used for SIMR-1 IP and wild-type IP, and anti-GFP conjugated beads were used for HRDE-2 IP and wild-type IP. The IPs with anti-HA and anti-GFP beads from wild-type samples had the same results (0 peptide count for the SIMR-1, HRDE-2, HRDE-1, and NRDE-3 proteins). Full protein lists are in Supplementary Data 1. b Western blot of HRDE-2 and HRDE-1 following HRDE-1 immunoprecipitation from the GFP::3xFLAG::HRDE-1; HRDE-2::2xTy1::GFP strain, shows that HRDE-2 interacts with HRDE-1. The HRDE-2::2xTy1::GFP strain was used as negative control. Anti-FLAG conjugated beads were used to immunoprecipitate GFP::3XFLAG::HRDE-1. Anti-FLAG, anti-Ty1, and anti-Actin antibodies were used to detect HRDE-1, HRDE-2, and Actin, respectively. This co-immunoprecipitation and western blot have been repeated three times with the same result. Source data are provided as a Source Data file.
