Fig. 6: Spermatogenic genes are de-silenced in a hrde-2 mutant.

a Normalized mRNA reads in hrde-2 mutants compared to wild-type from day 1 adults. WAGO-target and CSR-target genes are indicated in red and blue, respectively. One representative replicate is shown. b Normalized mRNA reads in hrde-2 mutants compared to wild-type from day 1 adults. Spermatogenic genes are indicated in green. One representative replicate is shown. c Box plots depicting log₂(fold change mRNA abundance) in hrde-1 and hrde-2 mutants compared to wild-type for three biological replicates. Bolded midline indicates median value, box indicates the first and third quartiles, and whiskers represent the most extreme data points within 1.5 times the interquartile range, excluding outliers. Two-tailed t-tests were performed to determine statistical significance and p values were adjusted for multiple comparisons. Adjusted p values are a comparison of the indicated gene list to all genes in the hrde-2 mutant. d Scatter plot displaying each gene as a function of its log₂ fold change for the level of small RNAs immunoprecipitated by HRDE-1 (x-axis) and mRNA expression (y-axis) in hrde-2 mutant compared to wild-type. WAGO class and CSR class genes are indicated in red and blue, respectively. e Scatter plot displaying each gene as a function of its log₂ fold change for the level of small RNAs immunoprecipitated by HRDE-1 (x-axis) and mRNA expression (y-axis) in hrde-2 mutant compared to wild-type. Spermatogenic genes are indicated in green.