Fig. 4: Loss of BCL7A/B impairs BRM-driven chromatin accessibility.

a Metagene plot reflecting substantially diminished accessibility at 11,644 accessible sites in WT upon loss of BRM. b Scatter plot showing fold-change of accessible peaks between WT and brm-1. Blue dots, stable peaks; pink dots, differential peaks. The numbers of differentially accessible peaks (increased or decreased) according to FDR are indicated. Heatmap (c) and metagene plots (d) reflecting the ATAC-seq signals over the retained, reduced, or increased chromatin accessibility sites of brm-1 in WT, bcl7a bcl7b, and brm-1. e Scatter plot showing fold-change of accessible peaks between WT and bcl7a bcl7b. Blue dots, stable peaks; pink dots, differential peaks. The numbers of differentially accessible peaks (increased or decreased) are indicated. f Venn diagram showing overlap between the sites with reduced accessibility in brm-1 compared with WT and those in bcl7a bcl7b. P values were calculated by the hypergeometric tests. g Scatter plot showing the correlation of chromatin accessibility changes (log₂(fold change)) between brm-1 (x-axis) and bcl7a bcl7b (y-axis) at a merged set of all accessible regions. R values are indicated. In both mutants, most affected regions show a comparable decrease in accessibility. h Box plots showing read counts at regions that showed reduced accessibility in brm-1 for the indicated ATAC-seq experiments. In box plots, center line and bounds of box represent median value and the interquartile range (IQR), respectively. Whiskers extend within 1.5 times the IQR. P values were calculated with the two-tailed Student’s t test. i Examples of ATAC-seq tracks at representative loci in the WT, bcl7a bcl7b, brm-3 and brm-1 mutants. Heat map (j) and metagene plots (k) reflecting BRM occupancy signals over sites that retained, reduced, or increased chromatin accessibility in brm-1 upon depletion of BCL7A/B. Profile plots (l) and Box plots (m) showing the mean accessibility signals of the indicated samples at the genes with decreased accessibility specifically identified in brm-1. In box plots, center line and bounds of box represent median value and the interquartile range (IQR), respectively. Whiskers extend within 1.5 times the IQR. P values were calculated with the two-tailed Student’s t test. Source data are provided as a Source Data file.