Fig. 2: Analysis of the cellular content of the day-5 aggregates.
From: In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells
a Uniform manifold approximation and projection of scRNA-seq data from the day-5 aggregates. Cluster number was determined according to the cluster size. Endo endoderm, NCC neural crest cell, NEC neuroepithelial cell, NNE/PPE non-neural ectoderm/pre-placodal epithelium, NPB neural plate border. b Dot plot of key marker genes to define each cluster. c Ratio of the annotated populations in the day-5 aggregates. d–f Feature plots of genes which characterize NCC-like cells and their progenitors g Violin plots of SOX10, PAX7, FOXD3, and SP5 (h–j) Feature plots of genes characterize immature cells, endo-like cells, NNE/PPE-like cells, NCC-derived neuron-like cells, NEC-like cells and placode-derived neuron-like cells.
