Fig. 1: DNL is increased in e/gWAT of K43M mice and is reversed upon ablation of RUNX1.
From: CDK6 inhibits de novo lipogenesis in white adipose tissues but not in the liver
Mice at the age of 6-8 months were i.p. injected with 10 µCi of D-[14C(U)]-glucose. The D-(U-14C)-glucose counts in the blood a were evaluated at 5-, 10-, 30-, and 60-min post-injection of D-[14C(U)]-glucose. Bar graphs shown a are the average of D-(U-14C)-glucose counts from 4 different time points. For male, WT (n = 10), K43M (n = 12), and KR (n = 3). For female WT/ K43M (n = 8), and KR (n = 3). At the end of experiments, e/gWAT were harvested and extracted to determine D-(U-14C) incorporation into TG b, c. Data were shown b, c as mean ± SE. For b, WT (n = 10), K43M (n = 12), and KR (n = 3); for c, WT/ K43M (n = 8), and KR (n = 3). d–f Serum concentrations of TG d, cholesterol e, and FFA f. g–i hepatic concentrations of TG g, cholesterol h, and FFA i. Data were shown as mean ± SE. For d–f, WT (n = 5), K43M (n = 4); for g–i, WT and K43M (n = 5). We calculated statistical significance using two-tailed Student’s T-test, with p < 0.05 considered significant. The p values were summarized above two compared groups. NS indicates no significance between two groups.
