Fig. 2: GLUT1, CHREBP and DNL enzymes are differentially regulated in WAT and liver in K43M mice.
From: CDK6 inhibits de novo lipogenesis in white adipose tissues but not in the liver
a, b, g, h Representative immunoblots of the indicated protein in eWAT or liver from 100 μg of cell lysates of male WT, K43M, and KR mice at the age of 18 weeks. α-tubulin was used as loading control. Three-five independent experiments were repeated with similar results. c, d, i, j Bar graphs summarizing fold changes of different protein expression from 3 to 5 independent experiments. The intensity of each protein was measured by FluorChem M system and then normalized to α-tubulin. The fold change of each protein was normalized to the WT control, which was arbitrarily set to 1 unit. Data were shown as mean ± S.E (n = 3–5 for different groups). e, f Relative mRNA expression levels of Glut1, and DNL-specific markers (Acly, Acc1, Fasn, and Scd1) of eWAT e and liver f tissues from male WT, K43M, and KR mice (n = 5–7 for different groups). The fold changes of each mRNA were normalized to the WT control, which was arbitrarily set to 1 unit. Data were shown as mean ± SE. *, We calculated statistical significance using two-tailed Student’s T-test, with p < 0.05 considered significant. P values were displayed above two groups. NS indicates no significance between two groups.
