Fig. 6: CDK6 interacted with and phosphorylated AMPKα and CHREBP.
From: CDK6 inhibits de novo lipogenesis in white adipose tissues but not in the liver
a–d IP-Western. The same amount (1 µg) of non-immune rabbit IgG and IgG of CHREBP a, CDK6 b, proteins containing CDK phosphorylated consensus motifs (p-T*PXK/R) c, and AMPKα d were used to immunoprecipitate eWAT extracts and blotted with the respective antibodies for CHREBP a, CDK6 b, T*PXK/R c, and AMPKα d. HC indicates a heavy chain of IgG. e–i IP-Westerns. AMPKα, CDK6, CHREBP, p-AMPKα, or proteins containing consensus CDK phosphorylation motif (p-T*PXK/R) was immunoprecipitated from eWAT extracts and blotted with the indicated antibodies. The Input represented 50 μg extracts from WT eWAT. Three-four independent experiments were repeated with similar results.
