Fig. 5: Immune subtypes of STS.
From: Proteomic characterization identifies clinically relevant subgroups of soft tissue sarcoma
A The heatmap illustrates enriched cell type compositions, proteins and pathways among three immune clusters. B The Kaplan-Meier curve illustrate distinguished OS of three immune clusters. Log-rank test is used for survival analysis. C The density contour plot of different immune clusters based on stroma and immune scores. D The bar plot indicates the proportion of proteomic clusters in each immune cluster. Per group number: IM-S-1 (PC-Ra = 33, PC-Cc = 13, PC-Sm = 50), IM-S-2 (PC-Ra = 21, PC-Cc = 66, PC-Sm = 6), IM-S-3 (PC-Ra = 32, PC-Cc = 43, PC-Sm = 8). E The bar plot indicates the proportion of patients with and without metastasis in each immune cluster. The number for each group: IM-S-1 (metastasis = 13, non-metastasis = 73), IM-S-2 (metastasis = 26, non-metastasis = 52), IM-S-3 (metastasis = 29, non-metastasis = 39). Fisher’s exact test is used for statistical analysis. F Boxplots present CD274 and CD80 abundance in different immune clusters. G Boxplots present CD274 and CD80 abundance in patients with and without metastasis of IM-S-3. Per group number: metastasis (n = 41) and non-metastasis (n = 40). The two-sided student’s t test is used for statistical analysis. H Boxplots present CD274 and CD80 abundance in different proteomic clusters. I The heatmap shows the metastasis ratio in different proteomic and immune clusters. J Boxplot presents CD274 and CD80 abundance in three groups: PC-Ra-nIM3 (n = 54), IM3-nPC-Ra (n = 51), and PC-Ra-IM3 (n = 32). K Boxplot presents phosphosite abundance of CTNNB1 Ser675 in three groups: PC-Ra-nIM3 (n = 18), IM3-nPC-Ra (n = 25), and PC-Ra-IM3 (n = 18). L Scatter plots present the correlation between CTNNB1 pSer675 and CD274 or CD80 abundance. M The boxplot shows log2-transformed MAPK10 protein abundance across PC-Ra-nIM3 (n = 54), IM3-nPC-Ra (n = 51), and PC-Ra-IM3 (n = 32). N Scatter plots present the correlation between MAPK10 and CD274/CD80. O The diagram illustrates the mechanism that MAPK10 phosphorylate CTNNB1 Ser675 to regulate CD274, resulting in promote metastasis. For (F, H, J, K, M), the Kruskal−Wallis’s test is used for statistical analysis. For (F–H, J, K, M), the middle bar represents the median and the box represents the interquartile range. Bars extend to 1.5× the interquartile range. For (L, N), Pearson’s correlation is used for associated analysis. Source data are provided as Source Data files.
