Fig. 3: Changes in Wnt4 proestrus expression are concentrated in the uterine stroma. | Nature Communications

Fig. 3: Changes in Wnt4 proestrus expression are concentrated in the uterine stroma.

From: A common allele increases endometrial Wnt4 expression, with antagonistic implications for pregnancy, reproductive cancers, and endometriosis

Fig. 3

A RNAscope localized expression of Wnt4 in the luminal epithelium of both WT in TG lines during proestrus. Expression was also identified in the stroma for the transgenic line but was not detectable for the wildtype. Luminal epithelia (LE), glandular epithelia (GE), and stroma are indicated. Two biological replicates are shown for each line. Wnt4 is stained a light brown, and the nucleus is a light indigo. Scale bar in each image measures 20 µm. B The concentration of Wnt4 expression differences in the stroma were confirmed with qPCR of primary endometrial stromal fibroblast cells (6 independent samples for WT, 5 for TG; See Fig. S3 for cell purity). Statistical significance was evaluated by the Wilcoxon-Mann-Whitney rank sum test. Boxplots show the median (central line) and interquartile range (50% of the data fall within the box), with the whiskers covering the range of data (Q1 or Q3 + 1.5IQR).

Back to article page